Flow cytometry (FCM) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer.
Clinical Application:
1. Analysis and absolute count of lymphocyte subsets:T/B/NK;
2.Detection: ankylosing spondylitis;
3. Blood disease/lymphoma immunophenotyping;
4. Functional lymphocyte subsets analysis: Th1, Treg;
5. Count of CD34 hemopoietic stem cell;
6. PNH auxiliary diagnosis:CD55 , CD59 , FLARE,CD14 , CD15;
7. Multiple myeloma;
8. Platelets: count and immunophenotype analysis of platelets;
9. Count of Reticulocytes;
10. Minor residual disease monitor of acute leukemia;
11. DNA ploidy analysis;
Item |
Parameters |
Laser |
488nm solid |
Detector |
FSC,SSC,FL1-FL5 |
Fluorescence Channel |
FL1:525/50(FITC) FL2:585/40(PE)
FL3:630/30(PE-Texas Red/ECD/PI) FL4:697/60(PerCP/PE-Cy5/PE-Cy5.5)
FL5:780/60(PE-Cy7) |
Fluorescence sensitivity |
FITC ≤ 100 MESF;PE ≤ 50 MESF |
Fluorescence linearity |
≥ 0.99 |
FSC sensitivity |
≤ 1μm |
Test particle size |
0.5-50μm |
Carry Over |
≤ 0.5% |
Instrument CV |
FSC and all FL channel CV less than 2% |
FSC and SSC CV |
Can separate red blood cells and platelet, can separate white blood cells into 3 groups |
Test speed |
≥38000 cells/s |
Minimum Sample Vol |
90μL |
Measure Range |
Maximum Support 107 |
Threshold |
Double threshold, minimum cells fraction |
Fluorescence compensation |
Support auto online compensation, offline compensation and visualized compensation |
Item Template |
Support Item template, acquisition condition/ fluorescence compensation 1-click use |
Intake method |
Manual intake
Rotary Wheel auto-sampling with auto- mixing function:32tests/wheel, |
Liquid storage station |
sheath bottle, waste bottle, shutdown liquid bottle 2L
cleaning liquid bottle 0.5L |
Software |
English/Chinese |
PC |
Dell(CPU i5/8G DDR4/1T hard disk)+Dell 27 inches monitor |
Size/ Weight |
500mm*605mm*305mm(L*W*H),35KG |
|
|
|
|
|
|
|
|
|
|
|
|
|